Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7cc05159j

Synthesis and biological properties of triazole-linked locked nucleic acid.

We have synthesized and studied the biological and biophysical properties of triazole-linked ribo and xylo locked nucleic acid (LNA). The combination of LNA with the Isobe triazole linkage gave high binding affinity when incorporated at the 3' or 5' termini of oligonucleotides, but low binding affinity at internal positions. Antisense oligonucleotides (ASOs) and siRNAs containing triazole dimer...

Synthesis of triazole-linked morpholino oligonucleotides via CuI catalysed cycloaddition† †Electronic supplementary information (ESI) available: Full experimental details and copies of 1H, 13C, 31P NMR spectra for all compounds. See DOI: 10.1039/c6ob00007j Click here for additional data file.

Triazole-linked morpholino ((TL)MO) oligonucleic acids were synthesised using the Cu(I) catalysed (3 + 2) azide-alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the (TL)MO modification gives higher Tm values than the corresponding (TL)DNA modification.

Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6ra08765e Click here for additional data file.

A red-NIR fluorescent dye detecting nuclear DNA G-quadruplexes: in vitro analysis and cell imaging† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6cc08492c Click here for additional data file.

Aggregation, red-NIR emission and light-up upon nucleic acid G-quadruplex binding have been investigated for a prototype core-extended naphthalene diimide, which is capable of fast cellular entry and nucleolar localization. Both high-level colocalization with an anti-G-quadruplex antibody and nucleolin displacement reveal that the compound targets and thus makes visible nuclear DNA G-quadruplexes.

Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides.

Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3'-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric ...